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Proteintech p jnk
P Jnk, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+jnk/Phospho-JNK+(Tyr185)+Recombinant+antibody/pm42892544-67-17-19
Average 96 stars, based on 274 article reviews

p jnk - by Bioz Stars, 2026-11

96/100 stars

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Western Blot:

Article Title: Osteoblast-derived osteomodulin restrains osteoclastogenesis via ITGB8/RRM2-mediated reduction of mitochondrial respiration and mitochondrial ATP production
Article Snippet: Protein bands were visualized using the Amersham Imager 600 (GE) and quantified using ImageJ. .. Western blotting (WB) was performed with the following antibodies: anti-GAPDH (1:10,000, 60004-1-IG; Proteintech), anti-β-actin (1:10,000, 66009-1-IG; Proteintech), anti-cathepsin K (CTSK) (1:100, sc-48353; Santa Cruz), anti-NFATC1 (1:100, sc-7294; Santa Cruz), anti-matrix metallopeptidase 9 (MMP9) (1:1,000, 10375-2-AP; Proteintech), anti-RRM2 (1:100, sc-81850; Santa Cruz), anti-ITGB8 (1:1,000, 29775-1-AP; Proteintech), anti-OMD (1:1,000, ab154249; Abcam), anti-RUNX2 (1:1,000, 20700-1-AP; Proteintech), anti-collagen type I (1:1,000, 14695-1-AP; Proteintech), anti-ATP Synthase F1 Subunit Alpha (ATP5A1) (1:10,000, 14676-1-AP; Proteintech), anti-ubiquinol-cytochrome c reductase core protein 2 (UQCRC2) (1:10,000, 67547-1-Ig; Proteintech), anti-succinate dehydrogenase subunits B (SDHB) (1:10,000, 10620-1-AP; Proteintech), anti-cytochrome oxidase subunit IV (MTCO4) (1:1,000, 11242-1-AP; Proteintech), anti-NADH-ubiquinone oxidoreductase ASHI subunit (NDUFB8) (1:10,000, 14794-1-AP; Proteintech), anti-FLAG (1:10,000, 20543-1-AP; Proteintech), anti-HA (1:10,000, 51064-2-AP; Proteintech), anti-YAP (1:1,000, ET1608-30; HUABIO), anti-p-YAP (S127) (1:1,000, ET1611–69; HUABIO), anti-Smad2 (1:1,000, ET1604-22; HUABIO), anti-p-Smad2 (1:5,000, ET1612-32; HUABIO), anti-Smad3 (1:5,000, ET1607-41; HUABIO), anti-p-Smad3 (1:2,000, ET1609-41; HUABIO), anti-AKT (1:1,000, #9272, Cell Signaling Technology), anti-p-AKT (1:2,000, #4060, Cell Signaling Technology), anti-ERK (1:1,000, #9102, Cell Signaling Technology), anti-p-ERK (1:1,000, #4695, Cell Signaling Technology), anti-p38 (1:1,000, #9212, Cell Signaling Technology), anti-p-p38 (1:1,000, #4511, Cell Signaling Technology), anti-JNK (1:5,000, 51153-1-AP; Proteintech) and anti-p-JNK (1:1,000, 80024-1-RR; Proteintech). ..

Article Title: Osteoblast-derived osteomodulin restrains osteoclastogenesis via ITGB8/RRM2-mediated reduction of mitochondrial respiration and mitochondrial ATP production.
Article Snippet: Protein bands were visualized using the Amersham Imager 600 (GE) and quantified using ImageJ. .. Western blotting (WB) was performed with the following antibodies: anti-GAPDH (1:10,000, 60004-1-IG; Proteintech), anti-β-actin (1:10,000, 66009-1-IG; Proteintech), anti-cathepsin K (CTSK) (1:100, sc-48353; Santa Cruz), anti-NFATC1 (1:100, sc-7294; Santa Cruz), anti-matrix metallopeptidase 9 (MMP9) (1:1,000, 10375-2-AP; Proteintech), anti-RRM2 (1:100, sc-81850; Santa Cruz), anti-ITGB8 (1:1,000, 29775-1-AP; Proteintech), anti-OMD (1:1,000, ab154249; Abcam), antiRUNX2 (1:1,000, 20700-1-AP; Proteintech), anti-collagen type I (1:1,000, 14695-1-AP; Proteintech), anti-ATP Synthase F1 Subunit Alpha (ATP5A1) (1:10,000, 14676-1-AP; Proteintech), anti-ubiquinol-cytochrome c reductase core protein 2 (UQCRC2) (1:10,000, 67547-1-Ig; Proteintech), anti-succinate dehydrogenase subunits B (SDHB) (1:10,000, 10620-1-AP; Proteintech), anti-cytochrome oxidase subunit IV (MTCO4) (1:1,000, 11242-1-AP; Proteintech), anti-NADH-ubiquinone oxidoreductase ASHI subunit (NDUFB8) (1:10,000, 14794-1-AP; Proteintech), anti-FLAG (1:10,000, 20543- 1-AP; Proteintech), anti-HA (1:10,000, 51064-2-AP; Proteintech), anti-YAP (1:1,000, ET1608-30; HUABIO), anti-p-YAP (S127) (1:1,000, ET1611–69; HUABIO), anti-Smad2 (1:1,000, ET1604-22; HUABIO), anti-p-Smad2 (1:5,000, ET1612-32; HUABIO), anti-Smad3 (1:5,000, ET1607-41; HUABIO), anti-p-Smad3 (1:2,000, ET1609-41; HUABIO), anti-AKT (1:1,000, #9272, Cell Signaling Technology), anti-p-AKT (1:2,000, #4060, Cell Signaling Technology), anti-ERK (1:1,000, #9102, Cell Signaling Technology), anti-p-ERK (1:1,000, #4695, Cell Signaling Technology), anti-p38 (1:1,000, #9212, Cell Signaling Technology), anti-p-p38 (1:1,000, #4511, Cell Signaling Technology), anti-JNK (1:5,000, 51153-1-AP; Proteintech) and anti-p-JNK (1:1,000, 80024-1-RR; Proteintech). ..

Incubation:

Article Title: Multi-Targeted Therapeutic Mechanisms of Huangqi Guizhi Wuwu Decoction Against Rheumatoid Arthritis: An Integrated Approach Combining Serum Pharmacochemistry, Network Pharmacology, Metabolomics, and Experimental Validation.
Article Snippet: .. The blockage of membranes was conducted with 5% non-fat milk in TBST for 1.5 h at room temperature, and overnight incubation was conducted at 4 ◦C with primary antibodies: anti-c-Jun (Proteintech, Rosemont, IL, USA), anti-p38 (Proteintech), anti-phospho-p38 (Cell Signaling Technology, Danvers, MA, USA), anti-JNK (Proteintech), anti-phospho-JNK (Cell Signaling Technology), and anti-β-tubulin (Proteintech) (All 1:1000, β-tubulin 1:5000). .. After rinsing with TBST, a 1 h incubation of membranes was conducted with HRP-conjugated secondary antibodies (1:5000, Proteintech) at room temperature.

Article Title: Sequential delivery of sinigrin and dabigatran by an in situ self-stabilizing dynamic hydrogel attenuates intervertebral disc degeneration
Article Snippet: Prior to SDS-PAGE, protein concentrations were quantified using a BCA Protein Assay Kit (Beyotime, China). .. The membranes were then incubated overnight with anti-GAPDH (1:50 000, AC054, abclone, China), anti-Col2α1(1:500, A19308, abclone, China), anti-Acan (1:1000, A8536, abclone, China), anti-SOX9 (1:1000, A19710, abclone, China), anti-MMP13 (1:1000, A1606, abclone, China), anti-ADAMTS4 (1:500, A2525, abclone, China), anti-iNOS (1:1000, 18985-1-AP, proteintech, China), anti-COX2 (1:1000, 12375-1-AP, proteintech, China), anti-ERK (1:2000, 11257-1-AP, proteintech, China), anti-Phospho-ERK (1:1000, 28733-1-AP, proteintech, China), anti-JNK (1:500, 51153-1-AP, proteintech, China), anti-Phospho-JNK (1:1000, 80024-1-RR, proteintech, China), anti-p38 (1:1000, A4771, abclone, China), anti-Phospho-p38 (1:500, AP1508, abclone, China), anti-p65 (1:5000, A19653, abclone, China), anti-Phospho-p65 (1:500, AP0475, abclone, China), anti-IκBα (1:500, A1187, abclone, China), anti-Phospho-IκBα (1:500, AP0420, abclone, China), anti-AMPK (1:5000, A28094, abclone, China), anti-Phospho-AMPK (1:500, AP0432, abclone, China), The membrane was incubated with HRP-conjugated Goat anti-rabbit IgG (H + L) (1:50 000, AS014, rabbit monoclonal, Abclone, China) and immunoreactive proteins were detected using enhanced chemiluminescence. .. Images were captured using the Bio-Rad ChemiDoc Touch Imaging System, and protein band density was analyzed using ImageJ software.

Article Title: Role of connexin32 in zinc oxide nanoparticles induced hepatotoxicity.
Article Snippet: the hepatotoxicity of zinc oxide nanoparticles(ZnO NPs) is of great concern.. connexin32 (cx32), as a crucial mediator for various kinds of liver injuries, has little investigation on ZnO NPs-induced hepatotoxicity.. in the present study, small interfering RNa were performed to specific knockdown of cx32 in BRl-3a rat liver cells and aMl12 mouse liver cells.

Article Title: Multi-Targeted Therapeutic Mechanisms of Huangqi Guizhi Wuwu Decoction Against Rheumatoid Arthritis: An Integrated Approach Combining Serum Pharmacochemistry, Network Pharmacology, Metabolomics, and Experimental Validation
Article Snippet: .. The blockage of membranes was conducted with 5% non-fat milk in TBST for 1.5 h at room temperature, and overnight incubation was conducted at 4 °C with primary antibodies: anti-c-Jun (Proteintech, Rosemont, IL, USA), anti-p38 (Proteintech), anti-phospho-p38 (Cell Signaling Technology, Danvers, MA, USA), anti-JNK (Proteintech), anti-phospho-JNK (Cell Signaling Technology), and anti-β-tubulin (Proteintech) (All 1:1000, β-tubulin 1:5000). .. After rinsing with TBST, a 1 h incubation of membranes was conducted with HRP-conjugated secondary antibodies (1:5000, Proteintech) at room temperature.

Membrane:

Hemagglutination Assay:

Article Title: SENP6 Restrains NLRP3 Inflammasome Activation via DeSUMOylation-Driven K48-Linked Ubiquitination of NLRP3 in Acute Lung Injury
Article Snippet: Anti-NLRP3 (15101S), anti-caspase-1/p20 (3866S), anti-IL-1β/p17 (63124S), anti-NLRC4 (12421S), and anti-AIM2 (12948S) were from Cell Signaling Technology (MA, USA). .. Anti-β-actin (66009-1-Ig), anti-hemagglutinin (HA) (51064-2-Ap), anti-His (66005-1-Ig), anti-DDDDK/Flag (66008-3-Ig), anti-SUMO1 (10329-1-Ap), anti-SUMO2/3 (11251-1-Ap), anti-p65 (10745-1-Ap), anti-pp65 (82335-1-RR), anti-IκBα (10268-1-AP), anti-p-IκBα (82349-1-RR), anti-ERK1/2 (11257-1-AP), anti-p-ERK1/2 (28733-1-AP), anti-JNK (24164-1-AP), and anti-p-JNK (80024-1-RR) were purchased from Proteintech Group (Wuhan, China). .. Anti-ASC (R013826) and anti-Myc (LF-301S) were purchased from Epizyme Biotech (Shanghai, China).

Review